Asymmetric subcellular mRNA distributions have been observed in a variety of polar cell types ( 1–4). The cytoplasm is a tightly regulated space that accommodates countless parallel tasks in specialized compartments. Testing this predictor on native mRNA sequencing data showed good agreement between predicted and observed localization potential, suggesting that the rules uncovered by our MPRA also apply to the localization of native full-length transcripts. Based on our data, we devised machine learning models that were able to predict the localization behavior of novel reporter sequences. We characterized the interplay between RNA stability and localization and identified motifs able to bias localization towards neurite or soma as well as the trans-acting factors required for their action. Mapping the localization potential of >300 genes revealed two ways neurite targeting can be achieved: focused localization motifs and broadly encoded localization potential. Here, we combined subcellular transcriptomics and massively parallel reporter assays and tested ∼50 000 sequences for their ability to localize to neurites. However, it is largely unknown how transcript sorting works in a sequence-specific manner. In neurons, localization of specific mRNAs to neurites is essential for cellular functioning. Choose which group you would like to import your EDIS publications into.Asymmetric subcellular mRNA localization allows spatial regulation of gene expression and functional compartmentalization. Select “EndNote Import” from the filter list.ġ0. Clicking the “Browse” button and select the. You will be presented with the EndNote Basic import interface pictured below:Ĩ.

Select the “Collect” tab from the EndNote Basic main menu.ħ. (Give the file a name be sure to select “Text (.txt)” from the file type drop down menu. Above your results list, you will see an option to “Output your results in EndNote format” and a button labeled “Export.” Click the “Export” button to export in EndNote format.Ĥ. You should see the screen similar to the one displayed below:ģ. A similar process may be used for all '.txt' format imports to EndNote Basic.ġ. These directions are designed to assist IFAS faculty with preparing publication lists.

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